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The Maintenance of the Human Body Louse Pediculus Humanus Corporis through Complete Cycles of Growth by Serial Feeding through Artificial Membranes¹

Two colonies of normal human body lice have been successfully raised by serial feeding through artificial membranes on meals of sterile, defibrinated, hemolyzed human blood, with and without the addition of 10 units each of penicillin and streptomycin per milliliter. In the first instance the experiment was terminated after forty-eight days, when third generation nymphs were hatching and feeding. In the second experiment, second generation nymphs were feeding and developing normally at the time of its discontinuance on day 27.

¹ This work was supported in part by research grants from the National Foundation for Infantile Paralysis, the Division of Research Grants of the National Institutes of Health, U. S. Public Health Service, E553(C2), and the Research Laboratories of Chas. Pfizer and Co., Inc.

² Dr. Haddon was aided by a Fellowship from the National Foundation for Infantile Paralysis.