AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 49(5), 1993, pp. 636-640
Copyright © 1993 by The American Society of Tropical Medicine and Hygiene

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A Polymerase Chain Reaction System for Rapid Diagnosis of Scrub Typhus within Six Hours

Yasuyuki Sugita, Yuko Yamakawa, Kazuo Takahashi, Tetsuo Nagatani, Kenji Okuda AND Hiroshi Nakajima
Departments of Dermatology and Bacteriology, Yokohama City University School of Medicine, Yokohama, Japan

A rapid diagnostic system for scrub typhus (tsutsugamushi disease) was established using the polymerase chain reaction (PCR) combined with microplate hybridization. With this system, diagnosis can be made within six hours without the need for radioisotopes, electrophoresis, or membrane hybridization. The use of a PCR processor and DNA-precoated enzyme-linked immunosorbent assay plates allows a rapid and precise diagnosis to be easily made even in primary medical care clinics.






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Copyright © 1993 by the American Society of Tropical Medicine and Hygiene.