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Am. J. Trop. Med. Hyg., 48(3), 1993, pp. 332-347
Copyright © 1993 by The American Society of Tropical Medicine and Hygiene

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An Improved Microassay for Plasmodium falciparum Cytoadherence using Stable Transformants of Chinese Hamster Ovary Cells Expressing CD36 or Intercellular Adhesion Molecule-1

Thomas Hasler, Glenn R. Albrecht, Marie Rose Van Schravendijk, Joao C. Aguiar, Kerstin E. Morehead, Brittan L. Pasloske, Cynthia Ma, John W. Barnwell, Brian Greenwood AND Russell J. Howard
Laboratory of Infectious Diseases, DNAX Research Institute, Palo Alto, California; Department of Medical and Molecular Parasitology, New York University Medical Center, New York, New York; Medical Research Council Laboratories, Fajara, The Gambia

Stable transformants of Chinese hamster ovary (CHO) cell lines expressing high levels of human CD36 or intercellular adhesion molecule-1 (ICAM-1) have been produced as target cells for cytoadherence of Plasmodium falciparum-infected erythrocytes. An improved adherence microassay has been designed using small sample volumes and allowing convenient and reliable measurements on a large number of samples. The assay can be used both with purified proteins spotted on plastic and with the stably transformed CHO cell lines. The same assay plate can be evaluated either microscopically or by scintillation counting after use of 3H-hypoxanthine-labeled parasites. Using the microassay, functional expression of the transfected receptor molecules on CHO-CD36 and CHO-ICAM was confirmed using parasites with different cytoadherence phenotypes and cytoadherence inhibition experiments with a panel of anti-CD36 antibodies. The use of isolates from The Gambia confirmed the applicability of these assays for laboratory studies of these isolates.




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Copyright © 1993 by the American Society of Tropical Medicine and Hygiene.