AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 48(2), 1993, pp. 198-204
Copyright © 1993 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bradley, J. E.
Right arrow Articles by McReynolds, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bradley, J. E.
Right arrow Articles by McReynolds, L.

A Sensitive Serodiagnostic Test for Onchocerciasis Using a Cocktail of Recombinant Antigens

J. E. Bradley, K. R. Trenholme, A. J. Gillespie, R. Guderian, V. Titanji, Y. Hong AND L. McReynolds
Department of Biology, Imperial College of Science, Technology, and Medicine, London, United Kingdom; Hospital Vozandes, Quito, Ecuador; Department of Biochemistry, University of Yaounde, Yaounde, Cameroon; New England Biolabs, Beverly, Massachusetts

A specific serodiagnostic test for onchocerciasis has been a priority objective of the World Health Organization. Fragments of cDNA encoding Onchocerca volvulus antigens selected on the basis of their specificity for this parasite were subcloned into a protein purification and expression system. No individual recombinant antigen showed a high sensitivity in an enzyme-linked immunosorbent assay because of heterogeneity in the response of O. volvulus-infected individuals. However, a cocktail of three recombinant proteins showed 96% sensitivity with 100% specificity, compared with 99% sensitivity and only 59% specificity against a crude O. volvulus extract. The sensitivity of detection of individual antigens varied between sera taken from individuals from different geographic areas infected with O. volvulus, but when used as a cocktail, all but one of the microfilariapositive individuals from all the geographic areas studied were detected. Recombinant probes provide a practical basis for specific diagnostic tests for helminth infections.




This article has been cited by other articles:


Home page
 J. Clin. Pathol.Home page
J A Andrews, W J Bligh, P L Chiodini, J E Bradley, P N Nde, and R Lucius
The role of a recombinant hybrid protein based ELISA for the serodiagnosis of Onchocerca volvulus
J. Clin. Pathol., March 1, 2008; 61(3): 347 - 351.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
M. A. Rodriguez-Perez, C. Lizarazo-Ortega, H. K. Hassan, A. Dominguez-Vasquez, J. Mendez-Galvan, P. Lugo-Moreno, M. Sauerbrey, F. Richards Jr., and T. R. Unnasch
Evidence for Suppression of Onchocerca volvulus Transmission in the Oaxaca Focus in Mexico
Am J Trop Med Hyg, January 1, 2008; 78(1): 147 - 152.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Society of Tropical Medicine and Hygiene.