AJTMH ASTMH Job Mart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 48(1), 1993, pp. 50-57
Copyright © 1993 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schwartz, B. S.
Right arrow Articles by Wormser, G. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwartz, B. S.
Right arrow Articles by Wormser, G. P.

Entomologic and Demographic Correlates of Anti-Tick Saliva Antibody in a Prospective Study of Tick Bite Subjects in Westchester County, New York

Brian S. Schwartz, Robert B. Nadelman, Durland Fish, James E. Childs, Gilda Foreseter AND Gary P. Wormser
Department of Environmental Health Sciences, Division of Occupational Health, and Department of Immunology and Infectious Diseases, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland; Department of Medicine, Division of Infectious Diseases, and Department of Community and Preventive Medicine, Medical Entomology Laboratory, New York Medical College, Valhalla, New York

We measured anti-tick saliva antibody (ATSA) by enzyme-linked immunosorbent assay using whole sonicated Ixodes dammini salivary glands as antigen in subjects with 1) a recent and confirmed I. dammini (n = 100) or Dermacentor variabilis bite (n = 3), 2) erythema migrans (n = 15), 3) late-stage Lyme disease (n = 4), and 4) normal controls without a history of tick bites (n = 5). Tick bite subjects had three ATSA determinations over approximately six weeks. On the first ATSA measurement at a mean ± SD of 18.5 ± 19.8 hr after removal of the tick, the subjects bitten by I. dammini had a mean ATSA optical density value (95% confidence interval [CI]) of 0.264 (0.223, 0.305); the corresponding value in controls was 0.142 (0.115, 0.169). There was no consistent change in ATSA levels in individuals with time. Multiple linear regression indicated that tick engorgement (P < 0.01), subject age (higher ATSA with increasing age; P = 0.01), and subject sex (females > males; P = 0.03) were all independent predictors of ATSA levels. Logistic regression revealed that a bite by I. dammini that became engorged (defined as an engorgement index ≥ 3.4) was a risk factor for ATSA seropositivity (odds ratio [95% CI] = 6.2 [1.7, 21.8]). Finally, the ATSA test had a sensitivity of 0.81 and a specificity of 0.56 for a bite by I. dammini that became engorged. Overall, the data are further evidence that ATSA is a biologic marker of tick exposure, in that the engorgement index, a surrogate for tick saliva dose, was the strongest independent predictor of antibody response.




This article has been cited by other articles:


Home page
 CVIHome page
F. Alarcon-Chaidez, R. Ryan, S. Wikel, K. Dardick, C. Lawler, I. M. Foppa, P. Tomas, A. Cushman, A. Hsieh, A. Spielman, et al.
Confirmation of Tick Bite by Detection of Antibody to Ixodes Calreticulin Salivary Protein
Clin. Vaccine Immunol., November 1, 2006; 13(11): 1217 - 1222.
[Abstract] [Full Text] [PDF]

Home page
 Am J EpidemiolHome page
R. Malouin, P. Winch, E. Leontsini, G. Glass, D. Simon, E. B. Hayes, and B. S. Schwartz
Longitudinal Evaluation of an Educational Intervention for Preventing Tick Bites in an Area with Endemic Lyme Disease in Baltimore County, Maryland
Am. J. Epidemiol., June 1, 2003; 157(11): 1039 - 1051.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Society of Tropical Medicine and Hygiene.