AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 46(3), 1992, pp. 307-313
Copyright © 1992 by The American Society of Tropical Medicine and Hygiene

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Antibodies to a Recombinant Glutamate-Rich Plasmodium Falciparum Protein: Evidence for Protection of Individuals Living in a Holoendemic Area of Liberia

Birthe Hogh, Eskild Petersen, Morten Dziegiel, Kim David, Aloysius Hanson, Martin Borre, Arne Holm, Jens Vuust AND Soren Jepsen
Laboratory of Parasitology, Department of Infection-Immunology, Division of Biotechnology, Statens Seruminstitut, Copenhagen, Denmark; Liberian Institute for Biomedical Research, Robertsfield, Liberia; Chemical Institute, Royal Veterinary and Agricultural University, Copenhagen, Denmark

A Plasmodium falciparum antigen gene coding for a 220-kD glutamate-rich protein (GLURP) has been cloned, and the 783 C-terminal amino acids of this protein (GLURP489–1271) have been expressed as a ß-galactosidase fusion protein in Escherichia coli. The encoded 783 amino acid residues contain two areas of repeated amino acid sequences. Antibodies against recombinant GLURP489–1271, as well as against a synthetic peptide corresponding to GLURP899–916, and against a synthetic peptide representing the major glutamate rich repeat sequence from the P. falciparum ring erythrocyte surface antigen (Pf155/RESA) (EENV)6 were examined in 423 individuals (age range 30 days-78 years) living in a malaria holoendemic area of Liberia. In the 5–9-year-old age group, subjects with anti-GLURP489–1271 antibody concentrations greater than the mean value of the group had lower parasite densities than those with low antibody concentrations (P = 0.0151). High levels of anti-GLURP899–916 antibodies did not correlate with low parasite densities. However, high levels of anti-(EENV)6 antibodies were associated with significantly lower parasite densities in the 2–4-year-old age group (P = 0.0189). There was no correlation between the anti-GLURP489–1271 and anti-(EENV)6 antibody responses. The data provide indirect evidence for a protective role of antibodies reacting with recombinant GLURP489–1271 as well as with the synthetic peptide (EENV)6 from the Pf155/RESA.




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J. T. BOUSEMA, W. ROEFFEN, M. VAN DER KOLK, S. J. DE VLAS, M. VAN DE VEGTE-BOLMER, M. J. BANGS, K. TEELEN, L. KURNIAWAN, J. D. MAGUIRE, J. K. BAIRD, et al.
RAPID ONSET OF TRANSMISSION-REDUCING ANTIBODIES IN JAVANESE MIGRANTS EXPOSED TO MALARIA IN PAPUA, INDONESIA.
Am J Trop Med Hyg, March 1, 2006; 74(3): 425 - 431.
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Copyright © 1992 by the American Society of Tropical Medicine and Hygiene.