AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 45(4), 1991, pp. 399-407
Copyright © 1991 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miller, B. R.
Right arrow Articles by Mitchell, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, B. R.
Right arrow Articles by Mitchell, C. J.

Genetic Selection of a Flavivirus-Refractory Strain of the Yellow Fever Mosquito Aedes Aegypti

Barry R. Miller AND Carl J. Mitchell
Medical Entomology-Ecology Branch, Division of Vector-Borne Infectious Diseases, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, US Department of Health and Human Services, Fort Collins, Colorado

Two inbred (isofemale) Aedes aegypti mosquito lines were derived that manifested a resistant or susceptible phenotype following ingestion of yellow fever virus; lack of virus movement from the midgut defined the resistant phenotype. Other flaviviruses, including dengue 1–4, Uganda S, and Zika, viruses behaved in a similar fashion in the two mosquito lines. Crosses between the two lines produced progeny that were of intermediate susceptibility, indicating codominance; F2 backcrosses to the parents yielded results consistent with a major controlling genetic locus and provide evidence of a second locus capable of modulating the phenotype of the major gene. The rapid selection necessary to fix the susceptible and refractory phenotypes support the hypothesis of a single major controlling locus. Viral movement across the midgut is likely to be governed by a single major gene and modifying minor genes or a group of closely linked genes. These inbred mosquito lines will be useful in discovering the molecular basis for flavivirus resistance in Ae. aegypti.




This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
S. HIGGS, D. L. VANLANDINGHAM, K. A. KLINGLER, K. L. MCELROY, C. E. MCGEE, L. HARRINGTON, J. LANG, T. P. MONATH, and F. GUIRAKHOO
GROWTH CHARACTERISTICS OF CHIMERIVAX-DEN VACCINE VIRUSES IN AEDES AEGYPTI AND AEDES ALBOPICTUS FROM THAILAND
Am J Trop Med Hyg, November 1, 2006; 75(5): 986 - 993.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
K. L. McElroy, K. A. Tsetsarkin, D. L. Vanlandingham, and S. Higgs
Role of the yellow fever virus structural protein genes in viral dissemination from the Aedes aegypti mosquito midgut.
J. Gen. Virol., October 1, 2006; 87(Pt 10): 2993 - 3001.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
D. L. VANLANDINGHAM, K. TSETSARKIN, K. A. KLINGLER, C. HONG, K. L. MCELROY, M. J. LEHANE, and S. HIGGS
DETERMINANTS OF VECTOR SPECIFICITY OF O'NYONG NYONG AND CHIKUNGUNYA VIRUSES IN ANOPHELES AND AEDES MOSQUITOES
Am J Trop Med Hyg, April 1, 2006; 74(4): 663 - 669.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. M. Attardo, S. Higgs, K. A. Klingler, D. L. Vanlandingham, and A. S. Raikhel
RNA interference-mediated knockdown of a GATA factor reveals a link to anautogeny in the mosquito Aedes aegypti
PNAS, November 11, 2003; 100(23): 13374 - 13379.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1991 by the American Society of Tropical Medicine and Hygiene.