AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 43(6), 1990, pp. 576-583
Copyright © 1990 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Collins, W. E.
Right arrow Articles by Wilson, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collins, W. E.
Right arrow Articles by Wilson, C. L.

Further Studies on the Immunization of Saimiri sciureus boliviensis with Recombinant Vaccines Based on the Circumsporozoite Protein of Plasmodium vivax

William E. Collins, Ruth S. Nussenzweig, Trenton K. Ruebush, II, Ian C. Bathurst, Elizabeth H. Nardin, Helen L. Gibson, Gary H. Campbell, Philip J. Barr, J. Roger Broderson, Jimmie C. Skinner, Valerie K. Filipski, Peggy S. Stanfill, Jacquelin M. Roberts AND Carla L. Wilson
Division of Parasitic Diseases and Office of Scientific Services, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia; New York University Medical Center, New York, New York; Chiron Corporation, Emeryville, California

Reported are the results of a trial in squirrel monkeys of 2 Plasmodium vivax malaria vaccine candidates based on the circumsporozoite (CS) protein, namely, rPvCs-2 and rPvCS-3. Compared with an earlier recombinant P. vivax CS construct, rPvCS-1, rPvCS-2 has an additional 24 amino acids at the C-terminal, which includes the thrombospondin region of homology and a putative T cell epitope. The rPvCS-3 was generated from a chemically synthesized gene that contained an additional 54 amino acids at the amino terminus and terminates at the same carboxy-terminal amino acid as rPvCS-2. In addition, rPvCS-3 contained only 1 each of the repeat sequences DRADGQPAG and DRAAGQPAG. Both antigens were administered with alum as adjuvant. Neither formulation caused toxic side effects and both recombinant molecules induced high antibody titers. Two monkeys were protected against sporozoite challenge by immunization with rPvCS-2 antigen, while none of the rPvCS-3 immunized animals displayed any degree of protection. While there was no correlation between protection and antibody titer or the in vitro proliferation of lymphocytes in response to the antigens, this is further evidence to support the role of the repeating epitopes in generating protective immunity.




This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
W. E. COLLINS, J. S. SULLIVAN, A. WILLIAMS, D. NACE, T. WILLIAMS, G. G. GALLAND, and J. W. BARNWELL
Aotus nancymaae as a potential model for the testing of anti-sporozoite and liver stage vaccines against Plasmodium falciparum.
Am J Trop Med Hyg, March 1, 2006; 74(3): 422 - 424.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by the American Society of Tropical Medicine and Hygiene.