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Am. J. Trop. Med. Hyg., 40(3), 1989, pp. 268-272
Copyright © 1989 by The American Society of Tropical Medicine and Hygiene

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Sensitive Determination of Circulating Anodic Antigen in Schistosoma mansoni Infected Individuals by an Enzyme-Linked Immunosorbent Assay using Monoclonal Antibodies

André M. Deelder, Niels De Jonge, Otto C. Boerman, Yvonne E. Fillié, Gerard W. Hilberath, J. Peter Rotmans, Marijke J. Gerritse AND D. W. O. Lex Schut
Laboratory of Parasitology, State University of Leiden, The Netherlands

From a panel of mouse monoclonal antibodies reactive with a repeating epitope of the schistosome circulating anodic antigen, an IgG1 monoclonal antibody was selected. This monoclonal antibody was applied in a sandwich enzyme-linked immunosorbent assay as capture antibody and as alkaline phosphatase labeled conjugate.

This assay allowed a sensitive quantitation of circulating anodic antigen in serum samples of infected individuals, detecting < 1 ng antigen/ml serum.

In Schistosoma mansoni infected individuals from Zaire, the level of antigen in serum correlated with fecal egg output. The lower detection level of the immunoassay corresponded to a level of about 10 eggs/gm feces.




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