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Comparison of Thick Films, in Vitro Culture and DNA Hybridization Probes for Detecting Plasmodium falciparum Malaria

David E. Lanar, Gerald L. McLaughlin^*, Dyann F. Wirth, Robert J. Barker, J. Werner Zolg AND Jeffrey D. Chulay

Walter Reed Army Institute of Research, Washington, DC 20307
^* University of Illinois, Urbana, Illinois 61801
Harvard School of Public Health, Cambridge, Massachusetts 02115
Biomedical Research Institute, Bethesda, Maryland 20852

Using blood from volunteers with sporozoite induced malaria, a comparison was made of the sensitivity and specificity of Giemsa stained thick film examination, in vitro culture, and 4 different DNA probes for detecting parasitemia. Between 9 and 13 days after sporozoite inoculation, patent parasitemia (4–550 parasites/µl) was detected by thick film examination of 0.5 µl blood in 7 volunteers. Cultures of 1 ml blood obtained 7 days after sporozoite inoculation were positive in all volunteers who eventually developed patent parasitemia. The DNA hybridization probes detected parasites in only 5–28% of smear- or culture-positive samples.

Accepted for publication September 23, 1988.