AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 4(5), 1955, pp. 863-871
Copyright © 1955 by The American Society of Tropical Medicine and Hygiene

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Tissue Culture Applied to the Study of Sindbis Virus1

Thomas E. Frothingham2
Department of Virology, U. S. Naval Medical Research Unit, Cairo, Egypt

1. The Ar-339 strain of Sindbis virus rapidly multiplies in plasma clot cultures of chick embryonic tissue and produces a marked cytopathogenic effect on fibroblast-like outgrowths. Similar changes are induced by the virus in cultures of human adult uterine tissue, and monkey testicle tissue. No cytopathogenic effect was observed in cultures of HeLa cells or in fibroblast-like outgrowths from human embryonic skin-muscle tissue.
2. On occasion fragments of chick embryo heart were observed to continue to rhythmically contract although the surrounding outgrowth of fibroblastic cells showed the typical degeneration.
3. A satisfactory neutralization test for the demonstration of neutralizing antibodies in human sera was developed, using cultures of embryonic chick cells. The results of comparative tests indicate that with Sindbis virus, chick embryonic tissue cultures are more practical for use in serological surveys than are infant mice.


1 This study was conducted under the auspices of the Ministry of Public Health of the Egyptian Government and the U. S. Naval Medical Research Unit No. 3 (NAMRU-3), with aid from The Rockefeller Foundation, and latterly from the Office of Naval Research through a contract administered by the University of Chicago.

The opinions or assertions contained herein are the private ones of the author and are not to be construed as official or reflecting the views of the Navy Department or the Naval Service at large.


2 Present address: Children's Hospital, Boston, Massachusetts.






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Copyright © 1955 by the American Society of Tropical Medicine and Hygiene.