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The Laboratory Diagnosis of Leptospirosis

by J. W. WOLFF, M.D., Institute of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands. 99 pp., illustrated. Springfield, Illinois: Charles C. Thomas, 1954. Price $3.75

This is a much needed text by a world-recognized authority on the leptospiroses. It is a monograph in one of the series of "American lectures in tests and techniques." It is printed in easy-to-read type on good paper and is attractively bound in a size that can readily be kept in a laboratory, where it will find its greatest usage.

The table of contents reveals sections on morphology and staining methods, diagnostic procedures including dark field examination, cultural procedures and media, animal inoculation and serologic procedures. Two sections are devoted to antigenic structures on leptospires and characteristics of type strains. These two chapters are especially important to all interested in leptospirosis.

The text is well organized and carefully written. The technics described are those that were developed by Dutch workers in the Netherlands and Indonesia over a period of twenty years. In general, the technics parallel those used by the American laboratories actively engaged in leptospiral research.