AJTMH ASTMH Job Mart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 39(5), 1988, pp. 469-471
Copyright © 1988 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Slemenda, S. B.
Right arrow Articles by Moore, D. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Slemenda, S. B.
Right arrow Articles by Moore, D. D.

Diagnosis of Paragonimiasis by Immunoblot

Susan B. Slemenda*, Shirley E. Maddison*, Elaine C. Jong{dagger} AND Deborah D. Moore{ddagger}
* Division of Parasitic Diseases
and{dagger} Division of Host Factors, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333,
and{ddagger} University of Washington, School of Medicine, Seattle, Washington 98195

A sensitive and specific immunoblot assay was used to rapidly and accurately diagnose paragonimiasis. The immunoreactivity of a complex Paragonimus westermani Chaffee antigen was evaluated by SDS-PAGE and Western blot analysis. Initial probing with pooled human serum from proven Paragonimus infections revealed many bands, including a significant antibody response to an approximately 8,000 molecular weight (8 kDa) protein. Forty-three of 45 proven paragonimiasis serum specimens had antibodies to this diagnostic band. Of 29 normal serum specimens and 210 serum specimens from patients with other parasitic and nonparasitic infections, only 1 serum, from a schistosomiasis haematobium patient, reacted positively. These results indicate that our immunoblot for paragonimiasis, which uses a comparatively crude antigen, is highly sensitive (96%) and specific (99%).

Accepted for publication May 4, 1988.




This article has been cited by other articles:


Home page
 NEJMHome page
E. T. Ryan, S. L. Aquino, and R. L. Kradin
Case 29-2007 -- A 51-Year-Old Man with Gastric Cancer and Lung Nodules
N. Engl. J. Med., September 20, 2007; 357(12): 1239 - 1246.
[Full Text] [PDF]

Home page
 ChestHome page
D. M. Boe and M. I. Schwarz
A 31-Year-Old Man With Chronic Cough and Hemoptysis
Chest, August 1, 2007; 132(2): 721 - 726.
[Full Text] [PDF]

Home page
 NEJMHome page
K. B. Robertson, W. J. Janssen, S. Saint, and S. E. Weinberger
The Missing Piece
N. Engl. J. Med., November 2, 2006; 355(18): 1913 - 1918.
[Full Text] [PDF]

Home page
 J Child NeurolHome page
A. Lowichik and A. J. Ruff
Topical Review: Parasitic Infections of the Central Nervous System in Children. Part III: Space-Occupying Lesions
J Child Neurol, May 1, 1995; 10(3): 177 - 190.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Society of Tropical Medicine and Hygiene.