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Am. J. Trop. Med. Hyg., 39(5), 1988, pp. 434-439
Copyright © 1988 by The American Society of Tropical Medicine and Hygiene

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Detection of Leishmania within Sand Flies by Kinetoplast DNA Hybridization

William O. Rogers, Paulo F. Burnheim* AND Dyann F. Wirth
Department of Tropical Public Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115,
and* Instituto de Medicina Tropical de Manaus, Avenida Dom Pedro Texeira, Manaus, Amazonas, Brazil, CEP 69.000

A kinetoplast DNA hybridization probe method was used to detect Leishmania within sand flies and to distinguish it from the non-pathogenic flagellate, Endotrypanum. Eighty-one sand flies (74 Lutzomyia umbratilis, 1 Lu. anduzei, and 6 Lu. shannoni) collected outside Manaus, Brazil were dissected. Forty-four of these were found to be infected with flagellates, and 2 hybridized with a Leishmania braziliensis probe. Thirty-three of sixty-one flies reprobed with an Endotrypanum probe were positive.

Accepted for publication March 22, 1988.






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Copyright © 1988 by the American Society of Tropical Medicine and Hygiene.