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A Comparative Field Study of Radiolabeled and Enzyme-Conjugated Synthetic DNA Probes for the Diagnosis of Falciparum Malaria

Orntipa Sethabutr^*, Arthur E. Brown, John Gingrich, H. Kyle Webster, Nopadol Pooyindee, David N. Taylor^* AND Peter Echeverria^*

^* Departments of Bacteriology
Departments of Immunology
Departments of Entomology, Armed Forces Research Institute of Medical Sciences, Bankok 10400, Thailand

Synthetic, 21-base, DNA probes to the genome of Plasmodium falciparum were either ³²P-labeled or enzyme-conjugated for comparative field studies. The sensitivity of both probes was compared with microscopy in the examination of blood samples from 97 Thai villagers, 47 Thai Rangers, and 19 malaria-free Bangkok residents. The probes were also used to monitor the therapeutic response of 18 of the Rangers during 7 days of treatment. The probes proved highly specific. Both probes had lower limits of detection of about 100 parasites per µl blood. Thus, the low parasite densities in partially immune villagers from an endemic area were often missed, while higher parasite densities in the nonimmune Rangers were usually detected. As monitors of response to treatment, the probes paralleled microscopy in identifying reversion from positive to negative parasitemia. The enzymelabeled DNA probe as shown to perform similarly to the radiolabeled probe in populations with different malarial immune status and during curative treatment.

Accepted for publication December 31, 1987.