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Am. J. Trop. Med. Hyg., 38(2), 1988, pp. 308-314
Copyright © 1988 by The American Society of Tropical Medicine and Hygiene

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The Use of Nonradioactive DNA Probes for the Characterization of Leishmania Isolates from Peru

Martin López*,{dagger},{ddagger},, Ysabel Montoya*,{dagger},, Margarita Arana*,{dagger},§,, Francisco Cruzalegui{dagger}, Janet Braga, Alejandro Llanos-Cuentas*,{dagger},, Guillermo Romero{dagger},{ddagger}, AND Jorge Arévalo*,{dagger},{ddagger},**,
* Centro de Investigación, Instituto Nacional de Salud,
{dagger} Instituto de Medicina Tropical "Alexander von Humboldt,",
{ddagger} Departments of Biochemistry
and§ Biology, Universidad Peruana Cayetano Heredia,
Universidad Nacional de la Amazonía,
and** Biochemistry Department, Universidad Nacional Federico Villarreal

We describe conditions for dot blot DNA hybridization studies using biotinylated kDNA probes from Leishmania. The sensitivity and specificity attained with biotinylated or 32P-labeled probes were equivalent. The lower level of detection obtained was 100 parasites that were blotted on nitrocellulose paper and then treated with Proteinase K. Studies were performed with 112 Leishmania isolates from Andean (uta) and sylvatic mucocutaneous (espundia) patients and all were determined to belong to the Leishmania braziliensis complex.

Accepted for publication September 4, 1987.






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Copyright © 1988 by the American Society of Tropical Medicine and Hygiene.