HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Martinez, S. Articles by Marr, J. J. Search for Related Content PubMed PubMed Citation Articles by Martinez, S. Articles by Marr, J. J.

A Tissue Culture System for the Growth of Several Species of Leishmania: Growth Kinetics and Drug Sensitivities

Samuel Martinez^*, Douglas L. Looker AND J. Joseph Marr

^* Departamento de Medicina Interna, Division Ciencias de la Salud, Universidad del Cauca, Popayan, Colombia, Sudamerica,
and Division of Infectious Diseases, Departments of Medicine and Biochemistry, University of Colorado Health Sciences Center, Denver, Colorado 80262

We have developed a simple in vitro method of infecting a continuous human macrophage cell line (U937) with promastigotes of several species of Leishmania. These include L. braziliensis braziliensis, L. b. panamensis, L. donovani, L. mexicana mexicana, L. m. pifanoi, L. tropica, and L. major. The growth kinetics of these species are presented as well as drug sensitivity data. The U937 cell system can be used to determine drug efficacy and eliminates the need to use amastigotes from animal tissues to infect the tissue culture.

Accepted for publication August 21, 1987.