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Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay (Elisa) for Detection of Plasmodium malariae Sporozoites in Mosquitoes

A monoclonal antibody specific for a repeated epitope of the circumsporozoite protein of Plasmodium malariae sporozoites has been used to develop a two-site, single antibody-based enzyme-linked immunosorbent assay that can detect P. malariae sporozoites in mosquitoes. The assay uses a purified monoclonal antibody produced against sporozoites of the Uganda I/CDC strain of P. malariae to capture the antigen and the same monoclonal antibody labeled with horseradish peroxidase as the detector. Sporozoites have been detected in laboratory-infected mosquitoes stored at room temperature in the presence of a desiccant for as long as 18 months. The detection limit of the assay is approximately 50 P. malariae sporozoites per test well. Cross-reaction has not been observed with mosquitoes infected with P. falciparum, P. vivax, or P. ovale sporozoites.

Accepted for publication September 1, 1987.

This article has been cited by other articles:

				W. E. Collins and G. M. Jeffery Plasmodium malariae: Parasite and Disease Clin. Microbiol. Rev., October 1, 2007; 20(4): 579 - 592. [Abstract] [Full Text] [PDF]