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Identification of Isomorphic Malaria Vectors using a DNA Probe

Sakol Panyim^*, Sukkid Yasothornsrikul^*, Sumalee Tungpradubkul^*, Visut Baimai, Ronald Rosenberg, R. G. Andre AND C. A. Green

^* Departments of Biochemistry and Biology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand
Walter Reed Army Institute of Research, Washington, DC 20307

About 7,000 recombinant clones, derived from chromosomally-identified families of wild-caught females of Anopheles dirus species D, were screened. The most promising clone was totally specific to species D when tested against single F₁ females of all four species of the complex. In fresh specimens the clone was positive for DNA levels 150 times less than the normal DNA content of single individuals. Fresh adult males and females, larvae, and dried specimens have been successfully identified. The clone was sequenced; it is 124 bp long and appears to be repeated in the genome about 1.8 x 10⁴ times.

Accepted for publication July 24, 1987.