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Monoclonal Antibodies Define Antigenic Variation in the ID Variety of Venezuelan Equine Encephalitis Virus

Rebeca Rico-Hesse^*,,, John T. Roehrig AND Robert W. Dickerman^*

^* Department of Microbiology, Cornell University Graduate School of Medical Sciences, 1300 York Avenue, New York, New York 10021
Division of Vector-Borne Viral Diseases, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of health and Human Services, P.O. Box 2087, Fort Collins, Colorado 80522

Three monoclonal antibodies were generated that are specific for the E2 glycoprotein of Venezuelan equine encephalitis (VEE) virus and have useful reactivities in an enzyme-linked immunosorbent assay (ELISA). Antibody 1A1B-9 distinguished between the IC (epizootic) and ID (enzootic) varieties of VEE virus by ELISA. Clone 7A1A-1 antibody distinguished the Panamanian prototype virus (3880) from Colombian ID isolates by a 500-fold difference in titer by endpoint ELISA, and it detected antigenic variation in ID isolates from southern Colombia and Ecuador. Antibody 7A3A-4 defined a cryptic antigenic site on the latter two isolates. These monoclonal antibodies complement others in identifying VEE isolates by a simple ELISA.

Accepted for publication June 29, 1987.