HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by de Water, R. Articles by Deelder, A. M. Search for Related Content PubMed PubMed Citation Articles by de Water, R. Articles by Deelder, A. M.

Schistosoma Mansoni: Ultrastructural Localization of the Circulating Anodic Antigen and the Circulating Cathodic Antigen in the Mouse Kidney Glomerulus

Ruud de Water^*, Erik A. E. Van Marck, Jack A. M. Fransen AND Andre M. Deelder^*

^* Laboratory for Parasitology, University of Leiden, Wassenaarseweg 62, NL-2333 Al Leiden, The Netherlands
Prince Leopold Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerpen, Belgium
Laboratory for Electron Microscopy, University of Leiden, Rijnsburgerweg 10, NL-2333 AA Leiden, The Netherlands

In this study two major antigens of Schistosoma mansoni, the circulating anodic antigen (CAA) and the circulating cathodic antigen (CCA), were localized ultrastructurally in glomeruli of S. mansoni infected mice. These antigens were studied by direct gold labeling in which anti-CAA and anti-CCA monoclonal antibodies were labeled with 5 and 15 nm gold particles, respectively. CAA and CCA were demonstrable in glomeruli at week 3 in the basement membrane and from 5 weeks in moderately electron-dense material of the mesangial matrix. Both antigens were also encountered in fenestrae of the endothelial cells, in filtration slit pores, and on the luminal membranes of the epithelial cells. It appears that CAA and CCA are arrested by the glomerular basement membrane and deposited in the mesangial matrix. CAA was seen in considerably smaller amounts than CCA. This was ascribed to the fact that CAA, but not CCA, is repelled by the negative charge of the capillary walls and the glomerular basement membrane.

Accepted for publication June 10, 1987.

This article has been cited by other articles:

				T. DeBrito, I. Nussenzveig, C. R. W. Carneiro, M. C. Nakhle, S. Carvalho, A. M. G. Silva, M. Sandoval, and L. B. Saldanha Glomerular Detection of Schistosomal Antigen by Immunoelectron Microscopy in Human Mansonian Schistosomiasis International Journal of Surgical Pathology, October 1, 1999; 7(4): 217 - 225. [Abstract] [PDF]