HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Beier, J. C. Articles by Hockmeyer, W. T. Search for Related Content PubMed PubMed Citation Articles by Beier, J. C. Articles by Hockmeyer, W. T.

Field Evaluation of an Enzyme-Linked Immunosorbent Assay (Elisa) for Plasmodium falciparum Sporozoite Detection in Anopheline Mosquitoes from Kenya

John C. Beier^*,,, Peter V. Perkins^*, Robert A. Wirtz, Richard E. Whitmire^*, M. Mugambi^* AND Wayne T. Hockmeyer

^* Biomedical Sciences Research Centre, Kenya Medical Research Institute, Nairobi, Kenya, U.S. Army Medical Research Unit-Kenya, Box 401, APO New York 09675,
and Departments of Immunology
and Entomology, Walter Reed Army Institute of Research, Washington, DC 20307-5100

An enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody that recognizes a repetitive epitope on the circumsporozoite protein of Plasmodium falciparum was used in Kenya to assess malaria infections in Anopheles gambiae s.l. and An. funestus. The ELISA confirmed that 88% of 44 sporozoite-positive gland dissections were P. falciparum. The ELISA infection rate of 18.6% (n = 736) for individually tested mosquitoes for both species was significantly higher than the 10.4% (n = 537) salivary gland sporozoite rate determined by dissection. This difference was due to ELISA detection of medium and large sized oocysts on the midguts of infected mosquitoes which did not contain salivary gland sporozoites. From a series of 379 Anopheles that were cut at the thorax, ELISA tests on "head" and "body" portions showed that 29.5% of 95 positive mosquitoes contained circumsporozoite antigen in the body portion in the absence of salivary gland infections. This field evaluation demonstrates that the ELISA can most accurately be used to estimate sporozoite rates by cutting mosquitoes at the thorax and testing anterior portions.

Accepted for publication November 18, 1986.

This article has been cited by other articles:

				M. Calzetta, F. Santolamazza, G. C. Carrara, P. J. Cani, F. Fortes, M. A. Di Deco, A. d. Torre, and V. Petrarca Distribution and Chromosomal Characterization of the Anopheles gambiae Complex in Angola Am J Trop Med Hyg, January 1, 2008; 78(1): 169 - 175. [Abstract] [Full Text] [PDF]

				A. Dicko, I. Sagara, D. Diemert, M. Sogoba, M. B. Niambele, A. Dao, G. Dolo, D. Yalcouye, D. A. Diallo, A. Saul, et al. Year-to-Year Variation in the Age-Specific Incidence of Clinical Malaria in Two Potential Vaccine Testing Sites in Mali With Different Levels of Malaria Transmission Intensity Am J Trop Med Hyg, December 1, 2007; 77(6): 1028 - 1033. [Abstract] [Full Text] [PDF]