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Specific Serodiagnosis with Adult Onchocerca volvulus Antigen in an Enzyme-Linked Immunosorbent Assay

I. Tada^*, M. Korenaga^*, K. Shiwaku, E. O. Ogunba, G. O. Ufomadu AND B. E. B. Nwoke^¶

^* Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan,
Department of Parasitology, Aichi Medical College, Aichi, Japan,
Department of Microbiology, University of Ibadan, Ibadan, Nigeria
Nigerian Institute for Trypanosomiasis Research, Vom, Nigeria,
and^¶ Department of Zoology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

The cross-reactivity of the blood from onchocerciasis, loiasis, and dipetalonemiasis was tested by a micro-ELISA technique, utilizing adult Onchocerca volvulus antigen and blood samples taken on filter paper. The average ELISA values (OD at 500 nm) were as follows: 0.58 in persons with O. volvulus microfilariae (n = 81), 0.49 in microfilariae-negatives from the same endemic area (n = 39), 0.15 in dipetalonemiasis (n = 27), and 0.25 in loiasis (n = 12), while those of 65 Dipetalonema perstans-negative people were markedly low (average 0.14) and that of 22 Loa loa-negatives, 0.22, respectively. This ELISA could successfully differentiate onchocerciasis from dipetalonemiasis and loiasis.

Accepted for publication October 6, 1986.