HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grimaldi, G. Articles by McMahon-Pratt, D. Search for Related Content PubMed PubMed Citation Articles by Grimaldi, G., Jr. Articles by McMahon-Pratt, D.

Identification and Distribution of New World Leishmania Species Characterized by Serodeme Analysis Using Monoclonal Antibodies

Gabriel Grimaldi, Jr.^*, John R. David AND Diane McMahon-Pratt

^* Department of Immunology, Instituto Oswaldo Cruz, Rio de Janeiro, R.J., Brazil,
Division of Tropical Medicine, Harvard Medical School, Boston, Massachusetts,
and Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut

Five hundred thirty stocks of Leishmania isolated from human and domestic and wild reservoir hosts, representing a wide geographic distribution of endemic foci of American cutaneous (ACL) and visceral leishmaniases (AVL) were characterized and identified at species and/or subspecies levels based on their reactivity to a cross-panel of specific monoclonal antibodies using a radioimmune binding assay. This study confirms and extends our preliminary results on the high specificity of some of these monoclonals for the L. braziliensis, L. mexicana, and L. donovani complexes. This study also demonstrates the relative stability of these molecular markers and the general usefulness of the method for parasite identification.

Two hundred ninety-two of 420 isolates of ACL were classified as members of the L. braziliensis complex. Two hundred twenty-seven were L. b. braziliensis; these showed the widest geographical distribution (Brazil: Amazonas, Bahia, Ceara, Espirito Santo, Goias, Minas Gerais, Para, Paraiba, Rio de Janeiro, and Sao Paulo; Honduras: Santa Barbara and Yoko; Peru: Ancash, Piura, and Ucayali; and Venezuela: Cojedes, Distrito Federal, Lara, Portuguesa, Vale Hondo, Yaracuy, and Zulia). Forty-one stocks were identified as L. b. guyanensis (from North Brazil: Amazonas, Amapa, Para, and Rondonia). Twenty-one stocks were identified as L. b. panamensis (from Costa Rica: Alajuela, Guanacasten, Limon, Puntarenas, and San Jose; and Honduras: El Paraiso, and Olancho). Out of 128 isolates classified as members of the L. mexicana complex, 74 were differentiated as L. m. amazonensis (from Bolivia; Brazil: Amazonas, Bahia, Ceara, Goias, Maranhao, Mato Grosso do Norte, and Para; Peru: Pasco Forest and Van Humboldt; and Venezuela: Carabobo, Guarico, and Merida). Forty-four stocks were identified as L. m. venezuelensis (from Venezuela: Lara). Six stocks were L. m. mexicana (from Belize; and Mexico: Michoacan and Quintana Roo, Yucatan). One hundred ten isolates from AVL were identified as L. donovani chagasi (from Brazil: Bahia, Ceara, Maranhao, Minas Gerais, Mato Grosso do Sul, Piaui, Rio de Janeiro, and Sergipe; and Honduras: Valle).

The implications of these results with respect to both the clinical and epidemiological data (including the detection of seven unusual characterized stocks) are discussed.

Accepted for publication September 2, 1986.

This article has been cited by other articles:

				I. Rodriguez-Barraquer, R. Gongora, M. Prager, R. Pacheco, L. M. Montero, A. Navas, C. Ferro, M. C. Miranda, and N. G. Saravia Etiologic Agent of an Epidemic of Cutaneous Leishmaniasis in Tolima, Colombia Am J Trop Med Hyg, February 1, 2008; 78(2): 276 - 282. [Abstract] [Full Text] [PDF]

				G. Bomfim, B. B. Andrade, S. Santos, J. Clarencio, M. Barral-Netto, and A. Barral Cellular Analysis of Cutaneous Leishmaniasis Lymphadenopathy: Insights into the Early Phases of Human Disease Am J Trop Med Hyg, November 1, 2007; 77(5): 854 - 859. [Abstract] [Full Text] [PDF]

				S. Ochsenreither, K. Kuhls, M. Schaar, W. Presber, and G. Schonian Multilocus Microsatellite Typing as a New Tool for Discrimination of Leishmania infantum MON-1 Strains J. Clin. Microbiol., February 1, 2006; 44(2): 495 - 503. [Abstract] [Full Text] [PDF]

				B. ROTUREAU ECOLOGY OF THE LEISHMANIA SPECIES IN THE GUIANAN ECOREGION COMPLEX Am J Trop Med Hyg, January 1, 2006; 74(1): 81 - 96. [Abstract] [Full Text] [PDF]

				J. Marfurt, A. Nasereddin, I. Niederwieser, C. L. Jaffe, H.-P. Beck, and I. Felger Identification and Differentiation of Leishmania Species in Clinical Samples by PCR Amplification of the Miniexon Sequence and Subsequent Restriction Fragment Length Polymorphism Analysis J. Clin. Microbiol., July 1, 2003; 41(7): 3147 - 3153. [Abstract] [Full Text] [PDF]

				T. M. Castilho, J. J. Shaw, and L. M. Floeter-Winter New PCR Assay Using Glucose-6-Phosphate Dehydrogenase for Identification of Leishmania Species J. Clin. Microbiol., February 1, 2003; 41(2): 540 - 546. [Abstract] [Full Text] [PDF]

				A. O. de Souza, F. P. Hemerly, A. C. Busollo, P. S. Melo, G. M. C. Machado, C. C. Miranda, R. M. Santa-Rita, M. Haun, L. L. Leon, D. N. Sato, et al. 3-[4'-Bromo-(1,1'-biphenyl)-4-yl]-N,N-dimethyl-3-(2-thienyl)-2-propen-1-amine: synthesis, cytotoxicity, and leishmanicidal, trypanocidal and antimycobacterial activities J. Antimicrob. Chemother., November 1, 2002; 50(5): 629 - 637. [Abstract] [Full Text] [PDF]

				E. H. G. Rodrigues, M. E. Felinto de Brito, M. G. Mendonca, R. P. Werkhauser, E. M. Coutinho, W. V. Souza, M. d. F. P. Militao de Albuquerque, M. L. Jardim, and F. G. C. Abath Evaluation of PCR for Diagnosis of American Cutaneous Leishmaniasis in an Area of Endemicity in Northeastern Brazil J. Clin. Microbiol., October 1, 2002; 40(10): 3572 - 3576. [Abstract] [Full Text] [PDF]