AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 36(2), 1987, pp. 257-263
Copyright © 1987 by The American Society of Tropical Medicine and Hygiene

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Simultaneous Use of Plasmodium falciparum Crude Antigen and Red Blood Cell Control Antigen in the Enzyme-Linked Immunosorbent Assay for Malaria

P. Demedts, C. Vermeulen-Van Overmeir AND M. Wery
Laboratory of Protozoology, Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium

An enzyme-linked immunosorbent assay for malaria, based upon duplicate testing of serum samples on both a crude Plasmodium falciparum antigen and a red blood cell control antigen, is evaluated. Results were analyzed using the Student's t-test for identification of positive serum samples (t ≥ 2.92, P ≤ 0.05) and for calculation of the mean difference in absorbance values ({Delta}ABS) obtained between the P. falciparum wells and the control wells. Cross-evaluation with the IFA test for P. falciparum antibodies gave 89.6% concording positive or negative results. Among discrepant sera 8.35% were ELISA+/IFA- and 2.05% ELISA-/IFA+. In addition, {Delta}ABS values in ELISA were highly correlated to titers obtained in immunofluorescence (r = 0.80, P < 0.001). The results confirm the high degree of species-specificity of the ELISA using P. falciparum crude antigen.

The necessity of the simultaneous use of red blood cell control antigen with a crude plasmodial antigen is demonstrated by comparing the presented results with those obtained on the P. falciparum antigen only.

Accepted for publication October 7, 1986.




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Copyright © 1987 by the American Society of Tropical Medicine and Hygiene.