AJTMH Tropical Medicine and Hygiene News
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 36(1), 1987, pp. 153-159
Copyright © 1987 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kuno, G.
Right arrow Articles by Gubler, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuno, G.
Right arrow Articles by Gubler, D. J.

Detecting Artificial Anti-Dengue IgM Immune Complexes Using an Enzyme-Linked Immunosorbent Assay

G. Kuno, I. Gómez AND D. J. Gubler
San Juan Laboratories, Dengue Branch, Division of Vector-Borne Viral Diseases, Center for Infectious Diseases, Centers for Disease Control, U.S. Public Health Service, GPO Box 4532, San Juan, Puerto Rico 00936

A variety of methods have been employed to detect viral immune complexes (IC) in clinical specimens. However, most techniques used were not antigen-specific. We developed a simple, specific double antibody sandwich technique to detect artificial anti-dengue (DEN) IgM immune complex (IgM-IC). Positive reactivity with IgM-ICs prepared with live DEN-1, -2, and -3 viruses was found to be related to IgM titers exceeding 1:20 and to the titer of the viruses. Most IgM-ICs prepared with live DEN-4 virus did not react. In contrast, IgM-ICs prepared with hemagglutination antigens, representing all 4 serotypes, reacted positively with amounts of antigens ranging from 2 to 8 units. These IgM-ICs were not type-specific.

Accepted for publication July 23, 1986.




This article has been cited by other articles:


Home page
 CVIHome page
P. Dussart, B. Labeau, G. Lagathu, P. Louis, M. R. T. Nunes, S. G. Rodrigues, C. Storck-Herrmann, R. Cesaire, J. Morvan, M. Flamand, et al.
Evaluation of an Enzyme Immunoassay for Detection of Dengue Virus NS1 Antigen in Human Serum
Clin. Vaccine Immunol., November 1, 2006; 13(11): 1185 - 1189.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
N. T. HUNG, N. T. LAN, H.-Y. LEI, Y.-S. LIN, L. B. LIEN, K.-J. HUANG, C.-F. LIN, D. Q. HA, V. T. Q. HUONG, L. T. MY, et al.
VOLUME REPLACEMENT IN INFANTS WITH DENGUE HEMORRHAGIC FEVER/DENGUE SHOCK SYNDROME
Am J Trop Med Hyg, April 1, 2006; 74(4): 684 - 691.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1987 by the American Society of Tropical Medicine and Hygiene.