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Identification of Brugia Malayi in Vectors with a Species-Specific DNA Probe

Betty Kim Lee Sim^*, Joon Wah Mak^**, Weng Hui Cheong^**, Inge Sutanto, Liliana Kurniawan, Haryani A. Marwoto, Eileen Franke, James R. Campell, Dyann F. Wirth^* AND Willy F. Piessens^*

^* Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts
^** Divisions of Filariasis and Malaria and Entomology, Institute for Medical Research, Kuala Lumpur, Malaysia
Department of Parasitology, University of Indonesia, Jakarta, Indonesia
National Institute of Health, Research and Development of the Ministry of Health, Jakarta, Indonesia
United States Naval Medical Research Unit No. 2, Jakarta Detachment, Jakarta, Indonesia

We evaluated the potential value of a cloned sequence of genomic DNA of Brugia malayi as a species-specific probe. Clone pBm 15 reacted with all stages of 8 different geographic isolates of B. malayi and cross-hybridized with microfilariae of B. timori. It did not hybridize with Wuchereria bancrofti or with B. pahangi, W. kalimantani, Dirofilaria repens, Breinlia booliati or Cardiofilaria species, animal filariids that can be sympatric with B. malayi. P³²-labeled clone pBm 15 correctly identified mosquitoes infected even with 1 infective larva of B. malayi. This specific DNA probe should be an invaluable tool to monitor control programs of Brugian filariasis.

Accepted for publication January 2, 1985.

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