HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ramsey, J. M. Articles by Mendez, J. F. Search for Related Content PubMed PubMed Citation Articles by Ramsey, J. M. Articles by Mendez, J. F.

Field Trial in Chiapas, Mexico, of a Rapid Detection Method for Malaria in Anopheline Vectors with Low Infection Rates

J. M. Ramsey^*, D. N. Bown^**, J. L. Aron, R. L. Beaudoin^* AND J. F. Mendez

^* Naval Medical Research Institute, Bethesda, Maryland 20814-5055
^** OPS, Apartado Postal 537, Tapachula, Chiapas, Mexico 30700
Biostatistics Branch, National Cancer Institute, Landow Building, Bethesda, Maryland 20205
Centro de Investigaciones de Paludismo, SSBA, Aparta Posta 537, Chiapas, Mexico 30700

A method consisting of filtration of up to 100 macerated mosquitoes in a batch, followed by fixation with glutaraldehyde and concentration of filtrate by centrifugation has been developed to rapidly assess malaria infection in anopheline mosquitoes. Determination of the presence of sporozoites is made by observation of a sample of the final filtrate with a phase microscope. The method is simple and field adaptable, essential factors for the application of any technique to large scale field operations. Application of the technique in El Gancho, Chiapas, Mexico, in February 1984 yielded an infection rate for Anopheles albimanus of 0.9% for intradomicile-collected human bait mosquitoes and 0.1% for peridomicile-collected human bait mosquitoes.

Accepted for publication November 6, 1985.