AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 35(1), 1986, pp. 135-140
Copyright © 1986 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Santiago, N.
Right arrow Articles by Morales, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Santiago, N.
Right arrow Articles by Morales, M. H.

Identification of Functional Fasciola hepatica Antigens in Experimental Infections in Rabbits

Noemi Santiago, George V. Hillyer, Miryam Garcia-Rosa AND Magda H. Morales
Laboratory of Parasite Immunology, Department of Biology, University of Puerto Rico, Rio Piedras, Puerto Rico 00931

Studies done comparing the reactivity of serum from an infected rabbit at different intervals of infection with a Fasciola hepatica crude tegument extract, F. hepatica excretory-secretory (ES) products and Fh are 2 by ELISA show that the first 2 antigen preparations offer a better sensitivity for detecting acute infection, especially in its early stages (3 weeks). The ES proteins from F. hepatica adult worms were identified by the enzyme-linked immunotransfer blot (EITB) in order to define the antigens recognized by the sera from rabbits with experimental fascioliasis. A group of 7 polypeptides with molecular weights of 23–28 Kd were the major antigens recognized. Reactivity to these antigens is maximum at 8–10 weeks of infection by EITB. As the infection progresses, reactivity to the 23–28 Kd antigens decreases but does persist through 52 weeks of infection. At least 5 other polypeptides of 120, 84, 58, 52, 39 and 33 Kd were recognized by the sera of infected rabbits from 6 to 52 weeks of infection. A different pattern was observed in parallel studies done with these same sera by immunoprecipitation of ES antigens. In addition to a 33 Kd antigen, which was detected by both techniques, a 62 Kd antigen was detected early in infection (5 weeks) and 3 major antigens of 38, 40 and 44 Kd were prominent by immunoprecipitation from 9 weeks onward. This implies the presence of multiple Fasciola antigens with serodiagnostic potential. The expression of these antigens during development and their possible role in immunity is discussed.

Accepted for publication September 26, 1985.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the American Society of Tropical Medicine and Hygiene.