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Am. J. Trop. Med. Hyg., 34(2), 1985, pp. 380-384
Copyright © 1985 by The American Society of Tropical Medicine and Hygiene

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Low Rates of Antigen Detection and Virus Isolation from the Peripheral Blood Leukocytes of Dengue Fever Patients*

S. H. Waterman, G. Kuno, D. J. Gubler AND G. E. Sather
Dengue Branch, Division of Vector-Borne Viral Diseases, Center for Infectious Diseases, Centers for Disease Control, U.S. Public Health Service, Department of Health and Human Services, GPO Box 4532, San Juan, Puerto Rico 00936

We evaluated direct fluorescent antibody (FA) testing of peripheral blood leukocytes (PBL) from patients in Puerto Rico with serologically and/or virologically confirmed dengue fever as a possible rapid diagnostic test and compared rates of dengue virus isolation from PBL with the rates from plasma or serum using the mosquito inoculation technique. Dengue antigen was detected in the PBL of only 1 of 19 patients with confirmed dengue. Virus was isolated from 3 of 19 PBL specimens and from 6 of 19 acute-phase serum or plasma samples. Four viruses were obtained from serum or plasma only and 1 isolate came from PBL only. We conclude that FA testing of PBL from dengue fever patients has little promise as a rapid diagnostic technique. Despite small numbers, our data suggest that virus isolation from PBL is less sensitive than that from serum or plasma. Our results differ considerably from those of previous studies of dengue hemorrhagic fever patients conducted in Thailand.

Accepted for publication August 23, 1984.


* Use of trade names and commercial sources is for identification only and does not imply endorsement by the Public Health Service or the U.S. Department of Health and Human Services.




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[Abstract] [Full Text] [PDF]


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Copyright © 1985 by the American Society of Tropical Medicine and Hygiene.