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Preparation of a New Antivenin by Affinity Chromatography

F. E. Russell^*, J. B. Sullivan^*, N. B. Egen^**, W. S. Jeter^*, F. S. Markland, W. A. Wingert AND D. Bar-Or

^* Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona
^** Biophysics Technology Laboratory, College of Engineering, University of Arizona, Tucson, Arizona 85721
School of Medicine, University of Southern California, Los Angeles, California 90033
Emergency Department, Denver General Hospital, Denver, Colorado 80204

Polyacrylamide gel affinity chromatography was employed to isolate and purify antibodies to the antigens of the venoms of four rattlesnakes. The antivenins were studied for their neutralizing properties on a number of pharmacologic preparations. It was found that the purified antibodies (IgG) were more efficacious than the commercially prepared antivenin in neutralizing the lethal, cytolytic, hemorrhagic, platelet aggregating, and other deleterious effects of the venoms. In addition, the purified antibodies gave no evidence of producing anaphylaxis or anaphylactoid reactions in animals sensitized to horse serum. The proposed technique is also more simple than current production methods, is time-saving, and less expensive.

Accepted for publication July 26, 1984.