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Am. J. Trop. Med. Hyg., 33(6), 1984, pp. 1173-1177
Copyright © 1984 by The American Society of Tropical Medicine and Hygiene

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Delayed Type Hypersensitivity Granuloma Formation Around Schistosoma Mansoni Eggs in Vitro

IV. Granuloma Formation in Human Schistosomiasis*

Barbara L. Doughty{dagger}, Deni M. Zodda{ddagger}, Aziz El Kholy AND S. Michael Phillips
University of Pennsylvania, School of Medicine, Department of Medicine, Allergy and Immunology Section, Philadelphia, Pennsylvania 19174, and The Biomedical Research Center for Infectious Diseases, Cairo, Egypt

Peripheral blood mononuclear cells, obtained from 42 school-age Egyptian children, were isolated on Ficoll-hypaque density gradients and assayed for granuloma formation. A granuloma index (G.I.) which classified the cellular reactions to Schistosoma mansoni eggs was determined for each patient. Morphologic criteria to assess the cellular reactivity included cell adherence, blast cell transformation, cell migration, and circumoval accumulation of inflammatory cells around the egg. The difference between the mean granuloma index of uninfected controls (G.I. = 1.25 ± 0.04) and infected patients (G.I. = 1.58 ± 0.05) was statistically significant (P < 0.01; Student's t-test). There was no correlation between the granuloma indices and infection intensities determined by quantitative egg counts or between anti-major serological antigen antibody titers. These data demonstrate the feasibility of studying granulomatous hypersensitivity in human schistosomiasis utilizing an in vitro model of granuloma formation and peripheral blood cells.

Accepted for publication March 8, 1984.


* Supported by grants from the National Institutes of Health, Bethesda (3 AI-15193), World Health Organization (B2/181/82), and The Rockefeller Foundation.


{dagger} Present address: Texas A&M University, College of Veterinary Medicine, Department of Veterinary Microbiology and Parasitology, College Station, Texas 77843.


{ddagger} Present address: Smith/Kline Diagnostics, Sunnyvale, California.






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