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Identification of Plasmodium Falciparum-Infected Mosquitoes by a Double Antibody Enzyme-Linked Immunosorbent Assay^*

T. R. Burkot, J. L. Williams AND I. Schneider

A double antibody micro enzyme-linked immunosorbent assay (ELISA) for identifying Plasmodium falciparum sporozoites in mosquitoes is described. Using monoclonal antibodies made against South American P. falciparum sporozoites, the ELISA was able to detect and identify sporozoite antigens of South American and Asian origins in extracts of dried infected mosquitoes.

Accepted for publication February 23, 1984.

^* The views of the author do not purport to reflect the position of the Department of the Army or the Department of Defense (para. 4-3, AR 360-5).

Present address: Papua New Guinea Institute of Medical Research, P.O. Box 378, Madang, Papua New Guinea.