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First Field Trial of an Immunoradiometric Assay for the Detection of Malaria Sporozoites in Mosquitoes

F. H. Collins^*,, F. Zavala, P. M. Graves, A. H. Cochrane, R. W. Gwadz, J. Akoh AND R. S. Nussenzweig

Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20205
Department of Microbiology, Division of Parasitology, New York University School of Medicine, New York, New York 10016
Department of Entomology, London School of Hygiene and Tropical Medicine, University of London, Keppel Street, London WC1E 7HT, United Kingdom

An immunoradiometric assay (IRMA) using a monoclonal antibody to the major surface protein of Plasmodium falciparum sporozoites was used to assess the P. falciparum sporozoite rate in a West African population of Anopheles gambiae (s.l.). Unlike current dissection techniques, the IRMA could detect sporozoite antigen in dried as well as fresh mosquitoes. In a controlled comparison, the sensitivity of the IRMA was comparable that of the dissection technique. Additionally, the IRMA was species specific and quantitative. Sensitivity of the assay was sufficient to detect sporozoite infections resulting from the development of a single oocyst.

Accepted for publication January 4, 1984.

^* Present address: Division of Parasitic Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia 30333.

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