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Long Term Cultivation of Plasmodium falciparum in Aotus trivirgatus Erythrocytes^*

The erythrocytes of the Colombian owl monkey Aotus trivirgatus griseimembra can be used for the long-term in vitro cultivation of Plasmodium falciparum employing a modified Trager-Jensen method. Cultures are grown in HEPES-buffered RPMI-1640 using a 4% suspension of monkey erythrocytes and 10% pooled heat-inactivated human AB serum, with initial parasitemias in a range between 0.2 and 0.5%. Adaptation of new strains from human erythrocyte cultures can be performed by simply subculturing from human to owl monkey erythrocytes in a stepwise manner. When 5% human AB serum is included in cultures to support growth, as much as 5% monkey serum can be added in order to investigate serum effects, such as antibody activity against P. falciparum. The Aotus trivirgatus continuous culture system has provided a stable, consistent source of infected erythrocytes for in vitro experimentation, and the techniques developed have been used to further refine and support the animal experiments in progress.

Accepted for publication November 2, 1984.

^* The opinions or assertions contained herein are the private views of the authors and should not be construed as official or as necessarily reflecting the views of the Uniformed Services University of the Health Sciences.

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				D Camus and T. Hadley A Plasmodium falciparum antigen that binds to host erythrocytes and merozoites Science, November 1, 1985; 230(4725): 553 - 556. [Abstract] [PDF]