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Identification of Malaria-Infected Mosquitoes by a Two-Site Enzyme-Linked Immunosorbent Assay

T. R. Burkot^*,, F. Zavala, R. W. Gwadz, F. H. Collins, R. S. Nussenzweig AND D. R. Roberts^*

^* Department of Entomology, Walter Reed Army Institute of Research, Washington, D.C. 20307
Division of Parasitology, Department of Microbiology, New York University Medical Center, New York, New York 10016
Malaria Section, Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20205

A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two-site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool.

Accepted for publication October 6, 1983.

Present address: Papua New Guinea Institute of Medical Research, P.O. Box 378, Madang, Papua New Guinea.

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