HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Strickland, G. T. Articles by Hunter, K. W. Search for Related Content PubMed PubMed Citation Articles by Strickland, G. T. Articles by Hunter, K. W.

Murine Malaria: Cellular Interactions in the Immune Response^*

G. Thomas Strickland, Aftab Ahmed, Peter C. Sayles AND Kenneth W. Hunter

The cellular and humoral interactions that contribute to protective immunity in Plasmodium yoelii malaria were studied by adoptive transfer of selective cell populations or hyperimmune serum into sublethally irradiated syngeneic C57BL/6 mice. For some experiments pools of mononuclear spleen cells were depleted of T or B lymphocytes and cells that take up silica were inactivated by standard procedures. Unfractionated immune spleen cells, but not nonimmune spleen cells, protected recipients from lethal P. yoelii challenge. Analysis of the protective capacity of subpopulations of immune spleen cells showed that levels of immunity similar to those seen after transfer of unfractionated immune cells were present only in those instances where immune macrophages, i.e., cells not previously inactivated with silica, were transferred concomitantly with either immune T (supplemented with nonimmune B) or immune B (supplemented with nonimmune T) cells. The requirement for immune macrophages could not be met by transferring mononuclear cells from a nonimmune donor. The results support the hypothesis that an immune 5,000 R-radioresistant, silica-inactivated, non-T, non-B cell, probably a macrophage, must act in concert with immune T and B lymphocytes in the optimal expression of transferred immunity to P. yoelii.

Accepted for publication March 25, 1983.

^* This work was supported by the Uniformed Services University of the Health Sciences Protocol R08305. The opinions or assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Uniformed Services University of the Health Sciences or Department of Defense. The experiments reported herein were conducted according to the principles set forth in the "Guide for the Care and Use of Laboratory Animals," Institute of Laboratory Animal Resources, National Research Council, DHEW Publ. No. (NIH) 7423.

Present address: International Health Program, University of Maryland School of Medicine, Baltimore, Maryland 21201. Send reprint requests to this address.

Present address: U.S. Naval Medical Research Unit #3, Cairo, Egypt.

Present address: Department of Preventive Medicine, New York State Veterinary College, Cornell University, Ithaca, New York 14850.