HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Urban, T. Articles by Aust-Kettis, A. Search for Related Content PubMed PubMed Citation Articles by Urban, T. Articles by Aust-Kettis, A.

Migration of Entamoeba Histolytica under Agarose^*

Teresa Urban, Connie Jarstrand AND Agneta Aust-Kettis

Department of Clinical Bacteriology of the Karolinska Institute at Roslagstulls Hospital, Stockholm
Department of Immunology, National Bacteriological Laboratory, Stockholm, Sweden

A procedure for visualizing and quantifying motility of Entamoeba histolytica by migration under agarose is described. Agarose suspended in tissue culture medium 199 supplemented with bovine albumin was poured into plastic dishes and allowed to harden. Six pairs of wells were cut out in a circular configuration. To the inner wells a suspension of E. histolytica in Eagle's Medium (24 x 10⁶ cells/ml) was added, and to the outer wells a chemoattractant or the control medium. After overnight incubation at 37°C, the amebae were fixed and stained. The chemotactic and spontaneous migrations were measured in an enlarging projector. Escherichia coli filtrates, suspensions of intact and lysed erythrocytes, and the complement factor C5a acted as good chemoattractants. Both the random and chemotactic motility were correlated to the time of the incubation. Cytochalasin B effected a dose-related inhibition of both chemotactic and random migration, while colchicine caused a decrease of the chemotaxis only. The reproducibility of the method, measured by 10 intra-assay tests, was good. Thus, the described method can be useful for comparative determinations of the motility of different ameba populations. Furthermore, different factors affecting the motility of amebae can be studied.

Accepted for publication December 16, 1982.

^* Address reprint requests to: Teresa Urban, Dr. Med. Sc., Department of Clinical Bacteriology of the Karolinska Institute at Roslagstulls Hospital, Box 5651, S-114 89 Stockholm, Sweden.

This article has been cited by other articles:

				M. Zaki, N. Andrew, and R. H. Insall Entamoeba histolytica cell movement: A central role for self-generated chemokines and chemorepellents PNAS, December 5, 2006; 103(49): 18751 - 18756. [Abstract] [Full Text] [PDF]

				S. Blazquez, C. Zimmer, G. Guigon, J.-C. Olivo-Marin, N. Guillen, and E. Labruyere Human Tumor Necrosis Factor is a Chemoattractant for the Parasite Entamoeba histolytica Infect. Immun., February 1, 2006; 74(2): 1407 - 1411. [Abstract] [Full Text] [PDF]