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Induced Division Synchrony in Entamoeba Histolytica

Effects of Hydroxyurea and Serum Deprivation^*

Synchronous growth of axenic Entamoeba histolytica was induced by nutritional deprivation and/or hydroxyurea. Axenic amebae maintained in 2% serum culture medium for 17–25 hours and returned to complete TP-S-1 (10% serum) medium showed a single synchronous cycle with a mean division time of 8.8 hours and a synchrony index of 0.47. Exposure to 10 mM hydroxyurea for 20–26 hours induced a single synchronous cycle with a division time of 3.0 hours and a synchrony index of 0.85. Hydroxyurea was toxic to 56% of the cells in the culture. The control generation time in TP-S-1 medium was 14.8 hours. Cells maintained for 44 hours in a nucleotide-deficient medium followed by a 10 mM hydroxyurea treatment exhibited two synchronous cycles. The first cycle had a generation time of 5.0 hours, a division time of 2.2 hours, and a synchrony index of 0.84.

Accepted for publication August 20, 1982.

^* A preliminary report of this work was presented before the annual meeting of the American Society of Tropical Medicine and Hygiene in San Juan, Puerto Rico, 16–19 November 1981, and before the annual meeting of the Federation of American Societies for Experimental Biology in New Orleans, Louisiana, 15–23 April 1982. Some of the data are from a thesis by C.J.A. submitted to the Graduate Faculty of the Louisiana State University Medical Center in partial fulfillment of the requirements of the Master of Science degree, 1980. The work was supported by Grant AI-15090 from the U.S. National Institutes of Health and Grant AI-02951.

Address reprint requests to: Dr. Lionel G. Warren, Department of Tropical Medicine and Medical Parasitology, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, Louisiana 70112.