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Am. J. Trop. Med. Hyg., 32(3), 1983, pp. 473-474
Copyright © 1983 by The American Society of Tropical Medicine and Hygiene

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The in Vitro Cultivation of Plasmodium Gonderi

Sheng-Chi Guo, William Chin AND William E. Collins
Parasitic Diseases Division, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333

Plasmodium gonderi, one of the vivax-type simian parasites, has been cultured in vitro by the candle jar method and by the sealed flask method. It showed a 48-hour asexual cycle and maintained its morphologic integrity. The effect of different sera (human, rabbit, and horse) was tested in culture and compared with results using rhesus serum. Growth was lower in rabbit and horse serum, but higher in human serum. Compared with individual human and rhesus serum, the multiplication rate in combined rhesus and human sera (1:1) was greater. In addition, human erythrocytes proved refractory to invasion by P. gonderi in vitro.

Accepted for publication August 27, 1982.






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Copyright © 1983 by the American Society of Tropical Medicine and Hygiene.