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Comparison of Microscopy and Culture in the Detection of Leishmania Donovani from Splenic Aspirates^*

Three culture media were compared with Giemsa-stained smears for the detection of Leishmania in splenic aspirates from Kenyan patients with visceral leishmaniasis. Ninety-nine splenic aspirates obtained from 26 patients at various times before, during, and after treatment were cultured in Schneider's Drosophila medium and RPMI medium 1640 (each supplemented with 20% fetal bovine serum) and McConnell's modification of Senekje's medium overlayed with 0.9% saline. From 13 splenic aspirates obtained before treatment, amastigotes were identified microscopically in all and promastigotes were cultured in 12. During and after treatment, Schneider's medium was the most sensitive method for detecting parasites, followed by microscopic examination of stained smears which was more sensitive than either of the other two media tested. Results indicate that, for initial diagnosis, both culture and direct microscopy of aspirates should be employed.

Accepted for publication August 13, 1982.

^* This is contribution number 1649 from the U.S. Army Research Program on Parasitic Diseases, and was supported in part by contract number DAMD17-82-G-9498 from the U.S. Army Medical Research and Development Command, and by the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. ADMB was a WHO consultant assigned to the Clinical Research Centre.

Address reprint requests to: Academic Affairs Branch, Walter Reed Army Institute of Research, Washington, D.C. 20012.

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