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Am. J. Trop. Med. Hyg., 32(1), 1983, pp. 114-117
Copyright © 1983 by The American Society of Tropical Medicine and Hygiene

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Hybridoma Antibody-Based Competitive Elisa in Schistosoma Japonicum Infection

Graham F. Mitchell, Robert R. Premier, Edito G. Garcia, John G. R. Hurrell, Howard M. Chandler, Kathy M. Cruise, Fe P. Tapales AND Wilfred U. Tiu
Laboratory of Immunoparasitology, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia Immunochemistry Research and Development Section, Commonwealth Serum Laboratories, Poplar Road, Parkville, Victoria 3052, Australia, and Department of Parasitology, Institute of Public Health, University of the Philippines System, Ermita, Manila 2801, Philippines

A competitive enzyme-linked immunosorbent assay (ELISA) has been developed and compared with the circumoval precipitin test (COPT) for diagnosis of schistosomiasis japonica using Philippine sera. The assay is based on the inhibition, by sera, of the binding of a penicillinase-conjugated hybridoma-derived antibody, I. 134, to a crude Schistosoma japonicum adult worm extract. A change in pH subsequent to addition of the substrate is used as the indicator system. Development of the color change in this assay is relatively slow, a fact which presumably facilitates detection of inhibition by serum. Relative to the COPT, no false positive reactions were obtained and the false negative rate was < 10%. A wide range of inhibitory titers was obtained using sera in the competitive ELISA similar to that found in a competitive radioimmunoassay using 125I-labeled I. 134. The competitive ELISA will be of more general application for diagnosis of schistosomiasis japonica than the competitive RIA using hybridoma antibodies, and will provide more precise quantitative information than is obtainable in the COPT.

Accepted for publication June 3, 1982.






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Copyright © 1983 by the American Society of Tropical Medicine and Hygiene.