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Cloning of African Trypanosomes in Mice Immunosuppressed by Cyclophosphamide Treatment^*

Abstract. The use of cyclophosphamide-treated mice for purposes of cloning the African trypanosomes was assessed. C3HeB/FeJ mice were injected with 200 mg/kg cyclophosphamide (CY) 24–72 hours prior to infection with a single trypanosome isolated from Trypanosoma rhodesiense infected blood samples. All CY-treated mice exhibited depressed parasite-specific and antigen nonspecific B lymphocyte responses for a period of 10 days after CY exposure, which was a period of time sufficient to grow trypanosomes from a single organism to a fulminating parasitemia. Cloning efficiency was routinely 45% in these animals. Thus, our study demonstrates that CY-treated mice are a convenient and efficient vehicle for cloning African trypanosomes. Techniques which facilitate the selection of single trypanosomes from infected blood are also described in this report.

Accepted for publication March 3, 1982.

^* Address reprint requests to: Dr. John M. Mansfield, Department of Microbiology and Immunology, School of Medicine, University of Louisville, Louisville, Kentucky 40292.

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				B. J. Leppert, J. M. Mansfield, and D. M. Paulnock The Soluble Variant Surface Glycoprotein of African Trypanosomes Is Recognized by a Macrophage Scavenger Receptor and Induces I{kappa}B{alpha} Degradation Independently of TRAF6-Mediated TLR Signaling J. Immunol., July 1, 2007; 179(1): 548 - 556. [Abstract] [Full Text] [PDF]