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The Cultivation in Vitro of Cells Derived from Adult Schistosoma Mansoni

I. Methodology; Criteria for Evaluation of Cultures; and Development of Media^*

We describe media and a cultural system that routinely supports development in vitro of plaques of several types of cells derived from adult Schistosoma mansoni. The results permit description of several types of cells observed in cultures and of criteria developed for the comparative evaluation of the supportive capacity of different media. Methods for the harvesting of worms, of their preparation for culture, and of the maintenance of cultures are detailed. The evolutionary development of the supportive media is summarized with comments on selected variables.

Accepted for publication July 8, 1981.

^* This study was initiated with the aid of NIH grant #AI-01023. For the past 6 years the research has been supported by grants from the Edna McConnell Clark Foundation. We gratefully acknowledge the contributions and innovative suggestions of Dr. Thomas M. Smith, formerly Associate Professor of Biochemistry, University of Mississippi Medical School, during his participation in this work while on sabbatical leaves in 1969 and 1974.