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Antigenic Differentiation of Trypanosoma Cruzi and Trypanosoma Rangeli by means of Monoclonal-Hybridoma Antibodies

Sera from Balb/c mice, hyperimmunized with ruptured epimastigotes of either Trypanosoma cruzi or Trypanosoma rangeli, lacked species-specificity when assayed for antibodies by indirect immunofluorescent microscopy and by the enzyme-linked immunosorbent assay. However, when plasma cells from those mice were fused with syngeneic mouse plasmacytoma cells, many of the resultant hybridomas synthesized antibodies which were species-specific. Four clones are synthesizing antibodies specific for antigens of T. rangeli. These antigens are associated with the cytoplasm, plasma membrane and flagellum. One clone is synthesizing a specific anti-T. rangeli antibody which appears to be reactive with the entire surface of the epimastigote. Another clone is reactive with a subpopulation of epimastigotes, thus suggesting some antigenic variability among cultured forms. Two clones are synthesizing antibody specific for antigens of T. cruzi, one of which is confined to the cytoplasm.

Accepted for publication February 28, 1981.