AJTMH ASTMH MEMBERSHIP INFORMATION: astmh@astmh.org
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 30(2), 1981, pp. 461-464
Copyright © 1981 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koff, W. C.
Right arrow Articles by Halstead, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koff, W. C.
Right arrow Articles by Halstead, S. B.

Propagation of Dengue Viruses in Murine Cell Cultures*

Wayne C. Koff, Joe L. Elm, Jr. AND Scott B. Halstead
Department of Tropical Medicine and Medical Microbiology, John A. Burns School of Medicine, University of Hawaii, 3675 Kilauea Avenue, Honolulu, Hawaii 96816

Dengue viruses were cultivated in Balb 3T12-3 cells, a continuous line of cells derived from mouse embryo cell cultures. Optimal serum concentrations were determined for maximal kinetics of virus replication. All four types of dengue viruses replicate in these cells, and peak virus titers were obtained by 72 hours post-infection. The successful growth of dengue viruses in a continuous line of mouse cells should allow for more extensive studies examining the cellular immune response to dengue virus infection in murine models.

Accepted for publication October 11, 1980.


* This work was supported by USPHS National Institutes of Health Grant No. 2 R01 HD08693.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1981 by the American Society of Tropical Medicine and Hygiene.