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Am. J. Trop. Med. Hyg., 30(2), 1981, pp. 449-460
Copyright © 1981 by The American Society of Tropical Medicine and Hygiene

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Isolation of Viruses from Female Culex Tritaeniorhynchus in Aedes Albopictus Cell Cultures*

Akira Igarashi, Kazuo Buei, Noboru Ueba, Masahiro Yoshida, Sumiyo Ito, Hiroshi Nakamura, Fuyoko Sasao AND Konosuke Fukai
Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Yamada-kami, Suita-city, Osaka, Japan 656, and Department of Public Health, Osaka Prefectural Institute of Public Health, No. 1-3-69, Nakamichi, Higashinari-ku, Osaka-city, Japan 537

Isolation of viruses from female Culex tritaeniorhynchus was performed by inoculation of specimens into Aedes albopictus clone C6/36 cells as well as into suckling mouse brains (SMB). Altogether, 32,812 mosquitoes in 349 pools were processed from the specimens collected in Osaka Prefecture during the summer of 1978. Thirty-nine strains of Japanese encephalitis virus (JEV) were isolated in C6/36 cells in contrast to 27 strains in SMB. Twentyfour pools yielded JEV both in cell cultures and in SMB, 10 yielded JEV only in cell cultures, and three only in SMB. Two strains of Getah (GET) virus were isolated in cell cultures but not in SMB. Two of the 23 plaque isolates of a GET strain showed significantly lower titers of plaque formation on BHK21 cells or SMB-LD50, compared with the titer of plaque formation on C6/36 cells at 28°C. In addition, many unidentified filterable agents were detected by plaque formation on C6/36 cells.

Accepted for publication November 17, 1979.


* Presented at the U.S.-Japan Intersociety Microbiology Congress, Honolulu, Hawaii, U.S.A., 8–11 May 1979.

Address reprint requests to: Dr. Akira Igarashi, Institute for Tropical Medicine, Nagasaki University, 12-4 Sakamoto-machi, Nagasaki, Japan 852.






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Copyright © 1981 by the American Society of Tropical Medicine and Hygiene.