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Am. J. Trop. Med. Hyg., 30(1), 1981, pp. 31-37
Copyright © 1981 by The American Society of Tropical Medicine and Hygiene

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The 48-Hour Exoerythrocytic Stage of Plasmodium Cynomolgi Bastianellii*

Wojciech A. Krotoski, William E. Collins, J. Roger Broderson, McWilson Warren AND Danuta M. Krotoski
Tropical Infectious Disease Research Program, Clinical Research Department, U.S. Public Health Service Hospital, New Orleans, Louisiana 70118, Vector Biology and Control Division, Bureau of Tropical Diseases and Scientific Services Division, Bureau of Laboratories, Center for Disease Control, Atlanta, Georgia 30333, and Biology Department, Tulane University, New Orleans, Louisiana 70118

Detection and specific identification of the 48-hour exoerythrocytic stage of the primate malaria parasite, Plasmodium cynomolgi bastianellii, was accomplished by means of a highly specific indirect immunofluorescence technique (IFA) applied to hepatic tissue fixed in Carnoy's solution. The 48-hour forms appeared as round-to-slightly-oval bodies of average mean diameter 3.0 µm (9 parasites) and lying within the cytoplasm of individual hepatic parenchymal cells; each possessed one to three non-fluorescent nuclei or nuclear sections (mean 1.6) within the brightly fluorescent parasitic cytoplasm. In contrast, 72-hour parasites (6) had an average mean diameter of 4.0 µm and a mean of 2.2 nuclei. Restaining of IFA preparations with the Giemsa-colophonium method confirmed the plasmodial nature of fluorescent forms, despite some modification of staining characteristics produced by the prolonged exposure of sections to the aqueous phase of the IFA procedure. Exoerythrocytic forms could not be detected in biopsies obtained 24 hours following sporozoite inoculation.

Accepted for publication August 9, 1980.


* Supported, in part, by Division of Hospitals and Clinics Grant ORL 78-27-76, Bureau of Medical Services, HSA, U.S. Public Health Service, Department of Health, Education and Welfare.




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J. SATTABONGKOT, N. YIMAMNUAYCHOKE, S. LEELAUDOMLIPI, M. RASAMEESORAJ, R. JENWITHISUK, R. E. COLEMAN, R. UDOMSANGPETCH, L. CUI, and T. G. BREWER
ESTABLISHMENT OF A HUMAN HEPATOCYTE LINE THAT SUPPORTS IN VITRO DEVELOPMENT OF THE EXO-ERYTHROCYTIC STAGES OF THE MALARIA PARASITES PLASMODIUM FALCIPARUM AND P. VIVAX.
Am J Trop Med Hyg, May 1, 2006; 74(5): 708 - 715.
[Abstract] [Full Text] [PDF]


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Copyright © 1981 by the American Society of Tropical Medicine and Hygiene.