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Inhibitory Effects of Immune Monkey Serum on Synchronized Plasmodium Falciparum Cultures^*

We studied the effects of heat-inactivated immune monkey serum on the growth of a partially synchronized culture of Plasmodium falciparum. By light microscopy, parasites within erythrocytes were morphologically indistinguishable from those cultured in normal serum. Immune serum reduced by 90% the number of erythrocytes containing newly invaded rings. Clusters of extracellular merozoites, usually around clumps of malarial pigment, were seen frequently in cultures grown with immune serum but rarely in cultures with normal serum. Electron microscopy confirmed the normal development of intraerythrocytic parasites. In immune serum cultures, electron-dense precipitates were found on the surface of schizonts, merozoites, and the excrescences on the plasma membrane of schizont-infected erythrocytes. Merozoites in immune serum cultures appeared to aggregate by adherence between adjacent surface coats. These findings support the hypothesis that immune serum agglutinates merozoites and thereby inhibits their invasion into uninfected erythrocytes.

Accepted for publication April 26, 1980.

^* This is contribution number 1568 from the Army Research Program on Malaria.

These data were presented, in part, at the 19th Interscience Conference on Antimicrobial Agents and Chemotherapy, Boston, Massachusetts, 1–5 October 1979.

In conducting the research described in this report, the investigators adhered to the Guide for the Care and Use of Laboratory Animals, as promulgated by the Institute of Laboratory Animal Resources, National Academy of Sciences, National Research Council.

Address correspondence to: Dr. Carter Diggs, Department of Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012.

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