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An Investigation into Hyaluronidase as a Factor in the Mechanism of Tissue Invasion by Endamoeba Histolytica

Neither intra- nor extracellular hyaluronidase was produced by E. histolytica in vitro when amebae were cultured aerobically or anaerobically. No specific hyaluronidase inhibitor was produced by the flora of any strains of ameba cultured in this laboratory. Production of hyaluronidase was not induced when cultures of amebae were seeded with hyaluronate. Amebae, isolated from liver abscesses in hamsters, failed to produce hyaluronidase under the conditions used.

¹ This work was sponsored by the Commission on Enteric Infections of the United States Armed Forces Epidemiology Board and supported by the Office of the Surgeon General, Department of the Army, Washington, D. C. Grateful acknowledgment is made to Mr. Peter J. Rennie for technical assistance and to the Allan Hancock Foundation of the University of Southern California for the use of certain of its facilities.